As we continue to work through the interest shown in our AP MALDI next generation sources at ASMS, we would like to highlight another paper that one of our employees (in collaboration with NIH/NIDA-IRP) presented.  Titled “High Resolution Tissue Imaging Using Sub-Atmospheric and Atmospheric MALDI Sources”, written by Eugene Moskovets, Shelley Jackson, Ludovic Muller, Damon Barbacci, Albert Schultz, Vladimir Doroshenko and Amina Woods, the work focused on the use of different matrices and different laser wavelengths to improve ion yield and study signal to noise ratio.  The poster showed 20 um (micron) resolution imagery

Conclusions (from the poster):

  • AP-MALDI MSI source coupled with the Orbitrap allowed the use of highly volatile matrices (i.e., DHA) efficient for imaging of neutral lipids
  • Sub AP-MALDI MSI source coupled with the ion funnel produces larger ion signals at the same laser fluences.  A use of the sub AP MALDI source reduces the time of MS imaging runs.
  • At atmospheric pressure, AgNPs were proven to be a very effective matrix for the analysis of cholesterol as a silver adduct and phosphatidylcholines (PC) and sphyingomyelins (SM) producing +H +Na and +K mass peaks.